Beyond Helper Phage: Using "Helper Cells" to Select Peptide Affinity Ligands
نویسندگان
چکیده
منابع مشابه
Beyond Helper Phage: Using "Helper Cells" to Select Peptide Affinity Ligands
Peptides are important affinity ligands for microscopy, biosensing, and targeted delivery. However, because they can have low affinity for their targets, their selection from large naïve libraries can be challenging. When selecting peptidic ligands from display libraries, it is important to: 1) ensure efficient display; 2) maximize the ability to select high affinity ligands; and 3) minimize th...
متن کاملEliminating helper phage from phage display
Phage display technology involves the display of proteins or peptides, as coat protein fusions, on the surface of a phage or phagemid particles. Using standard technology, helper phage are essential for the replication and assembly of phagemid particles, during library production and biopanning. We have eliminated the need to add helper phage by using 'bacterial packaging cell lines' that provi...
متن کاملMemory CD4+ T cells: beyond "helper" functions.
In influenza virus infection, antibodies, memory CD8+ T cells, and CD4+ T cells have all been shown to mediate immune protection, but how they operate and interact with one another to mediate efficient immune responses against virus infection is not well understood. In this issue of the JCI, McKinstry et al. have identified unique functions of memory CD4+ T cells beyond providing "help" for B c...
متن کاملPreparation of peptide-targeted phagemid particles using a protein III-modified helper phage.
Ligand or peptide-targeted phagemid particles are being pursued as vehicles for receptor-mediated gene delivery. Here we describe a helper phage in which the protein III (pIII) protein is modified by the addition of a ligand peptide sequence at the amino terminus. Phagemid particles can be prepared with the help of this modified helper phage and should display the ligand peptide in most of the ...
متن کاملHelper Cells in Vitro
The cellular immune reactions to hapten-protein conjugates (in the generation of hapten-specific primary and secondary humoral responses) display a significant dependence upon carrier recognition (reviewed in reference 1). Experimental models, particularly the adoptive transfer of immunocompetent cells into irradiated hosts (2-5), have established that the cooperative interactions involved requ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: PLOS ONE
سال: 2016
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0160940